Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain sections)
Specification
brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Other product details
Dilution
1/300
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST
Secondary antibody
Name
Dilution
1/500
Additional data
Additional Notes
Immunostaining for Ctip2 using the antibody reference ab 28448 1:300 diluted in the rat brain. The pictures show the staining obtained in the cortex (left panel, Objective X10) and hippocampus (right panel, Objective X20)). The staining is observed in subpopulation of neurons in cortical neurons in laminae IV and V, hippocampus and striatum. The staining is thinly punctuated, very pretty. IV, V and VI indicate the cortical laminae where the staining was observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
投稿 Aug 25 2008