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Immunofluorescence - Cryptochrome I antibody (ab54649)
Cryptochrome I antibody (ab54649) used in immunofluorescence at 10ug/ml on HeLa cells.
IHC-P - Cryptochrome I antibody (ab54649)
Cryptochrome I antibody (ab54649) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon adenocarcinoma tissue.
Western blot - Anti-Cryptochrome I antibody (ab54649)
Predicted band size : 66 kDa
Cryptochrome I antibody (ab54649) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry-Cryptochrome I antibody(ab54649)
Overlay histogram showing HeLa cells stained with ab54649 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54649, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Anti-Cryptochrome I antibody for Western blot in Mouse (54649)
Cryptochrome I antibody for ICC/IF in Human (54649)