Anti-Cpn10 抗体 (ab90494)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cpn10
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Rabbit, Guinea pig, Cow, Dog, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide derived from sequence near the C terminal of Saccharomyces cerevisiae Cpn10, conjugated to KLH.

  • ポジティブ・コントロール
    • HeLa cell lysate (heat shocked)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 11 kDa.

ターゲット情報

  • 機能
    Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
  • 配列類似性
    Belongs to the GroES chaperonin family.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 10 kDa chaperonin antibody
    • 10 kDa heat shock protein mitochondrial antibody
    • 10 kDa heat shock protein, mitochondrial antibody
    • CH10_HUMAN antibody
    • Chaperonin 10 antibody
    • Chaperonin 10 homolog antibody
    • CPN10 antibody
    • cpn10 homolog antibody
    • Early pregnancy factor antibody
    • Early-pregnancy factor antibody
    • EPF antibody
    • GROES antibody
    • GroES homolog antibody
    • Heat shock 10kD protein 1 chaperonin 10 antibody
    • Heat shock 10kDa protein 1 antibody
    • Heat shock 10kDa protein 1 chaperonin 10 antibody
    • Heat shock protein family E (Hsp10) member antibody
    • Heat-shock 10-kD protein antibody
    • Hsp10 antibody
    • Hspe1 antibody
    see all

画像

  • Anti-Cpn10 antibody (ab90494) at 1/2000 dilution + HeLa cell lysate (heat shocked)

    Predicted band size : 11 kDa

プロトコール

参考文献

ab90494 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your reply and I apologize for the delay as I was awaiting more information from the lab. They were unable to BLAST the sequence agains the rrequested species, but they did offer this information below:

Please see thelink below f...

Read More

Thank you for contacting us.

We do not make this antibody in-house and unfortunately the supplying lab considers the exact immunogen sequence to be proprietary. The only information we know is thatthe immunogen that was used to raise the ant...

Read More

Thank you for your inquiry.

This antibody is a polyclonal so it will have multiple binding epitopes. However, the immunogen that was used to raise the antibody corresponds to a sequence near the C terminal of Saccharomyces cerevisiae Cpn10. <...

Read More
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (uterus)
Loading amount
50 µg
Specification
uterus
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録