Cortisol ELISA Kit (ab108665)
Key features and details
- Sensitivity: 2.44 ng/ml
- Range: 10 ng/ml - 500 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Cortisol ELISA Kit
Cortisol キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 60 <=9% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 30 <=9.8% -
サンプルの種類
Urine, Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Competitive -
検出感度
= 2.44 ng/ml -
検出範囲
10 ng/ml - 500 ng/ml -
添加回収試験
103.56 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.
A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Washing Solution 1 x 50ml Anti-Cortisol (IgG) Coated Microplate (12 x 8 wells) 1 unit Cortisol Control 1 x 1ml Cortisol Standard 0 – 0 ng/mL 1 x 1ml Cortisol Standard 1 – 10 ng/mL 1 x 1ml Cortisol Standard 2 – 50 ng/mL 1 x 1ml Cortisol Standard 3 – 150 ng/mL 1 x 1ml Cortisol Standard 4 – 500 ng/mL 1 x 1ml Cortisol-HRP conjugate 1 x 21ml Stop Solution 1 x 15ml TMB Substrate Solution 1 x 15ml -
研究分野
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab108665 は 13 報の論文で使用されています。
- Morales-Lange B et al. Dietary Inclusion of Hydrolyzed Debaryomyces hansenii Yeasts Modulates Physiological Responses in Plasma and Immune Organs of Atlantic Salmon (Salmo salar) Parr Exposed to Acute Hypoxia Stress. Front Physiol 13:836810 (2022). PubMed: 35418880
- Inbaraj G et al. Impact of integrated yoga therapy on cognitive impairment and cardiac dysfunction in relation to quality of life in breast cancer patients undergoing chemotherapy: Study protocol for a two-arm randomized controlled trial. Front Oncol 12:955184 (2022). PubMed: 36185291
- Shaw J et al. Effectiveness of short-term isothermic-heat acclimation (4 days) on physical performance in moderately trained males. PLoS One 17:e0270093 (2022). PubMed: 36395344
- Djordjevic B et al. Comparison of Circulating Markers and Mucosal Immune Parameters from Skin and Distal Intestine of Atlantic Salmon in Two Models of Acute Stress. Int J Mol Sci 22:N/A (2021). PubMed: 33494146
- Chang M et al. Genistein acts as antidepressant agent against chronic mild stress-induced depression model of rats through augmentation of brain-derived neurotrophic factor. Brain Behav 11:e2300 (2021). PubMed: 34333865