Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Paraventricular nucleus brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Paraventricular nucleus brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Triton-x 1% goat serum in 0.1M PB
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: AF488 anti-mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Tested this antibody at dilutions ranging from 1/100 to 1/1600. The sections were free floating 60 micrometres thick, therefore a little thicker than ideal, nevertheless would have expected to see labelling. Control sections were incubated without primary antibody, but they looked the same as the test.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Fiona Shenton
Verified customer
投稿 Jun 26 2017