Corticosterone ELISA kit (ab108821)
Key features and details
- Sensitivity: 0.28 ng/ml
- Range: 0.391 ng/ml - 25 ng/ml
- Sample type: Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse, Rat, Human, Baboon
製品の概要
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製品名
Corticosterone ELISA kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 10.6% -
サンプルの種類
Saliva, Milk, Urine, Serum, Plasma -
アッセイタイプ
Competitive -
検出感度
= 0.28 ng/ml -
検出範囲
0.391 ng/ml - 25 ng/ml -
添加回収試験
= 98 %
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全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse, Rat, Human, Baboon
交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig -
製品の概要
Corticosterone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, and saliva samples.
A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.
Please note the concentrations of the kit components are lot-specific and the end user should always refer to the vial label
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 1 x 80µl 10X Diluent S Concentrate 1 x 20ml 1 x 20ml 2X Biotinylated Corticosterone Protein (Lyophilized) 1 vial 1 vial 20X Wash Buffer Concentrate 1 x 30ml 1 x 30ml Chromogen Substrate 1 x 7ml 1 x 7ml Corticosterone Microplate (12 x 8 well strips) 1 unit 1 unit Corticosterone Standard 1 vial 1 vial Sealing Tapes 3 units 3 units Stop Solution 1 x 11ml 1 x 11ml -
研究分野
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関連性
Corticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone.
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (134)
ab108821 は 134 報の論文で使用されています。
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- Rosen SF et al. Olfactory exposure to late-pregnant and lactating mice causes stress-induced analgesia in male mice. Sci Adv 8:eabi9366 (2022). PubMed: 35594354
- Poller WC et al. Brain motor and fear circuits regulate leukocytes during acute stress. Nature 607:578-584 (2022). PubMed: 35636458