Complex IV Rodent Enzyme Activity Dipstick Assay Kit (ab109878)
製品の概要
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製品名
Complex IV Rodent Enzyme Activity Dipstick Assay Kit
Complex IV キット 製品一覧 -
サンプルの種類
Cell culture extracts, Tissue -
アッセイタイプ
Sandwich (quantitative) -
種交差性
交差種: Mouse, Rat -
製品の概要
ab109878(MS432) contains 30 or 90 dipsticks and necessary components to quantify the activity of the cytochrome c oxidase enzyme complex from mouse and rat samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.
Using an antibody immobilized on the dipstick that specifically recognizes COX, the enzyme complex is immno-captured (i.e immuno-precipitated in active form) and enzyme activity is determined directly on the dipstick. Using a traditional method for determining COX enzyme activity by histo-chemical methods and in-gel activity assays, Di-amino benzidinetetrachloride (DAB) serves as the reporter of COX activity. The greater the signal of precipitated DAB, the greater the amount of COX enzyme complex. In addition, the reaction is cyanide sensitive. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system. -
特記事項
Store dipsticks at room temperature in their provided container and out of direct sunlight.
High humidity conditions should be avoided.
Store Buffer A, B, and C at 4°C or at -20°C for long term storage.
Store Tubes 1 and 2 at -20°C or at -80°C for long term storage; they can also be aliquoted upon receipt to prevent freeze/thaw cycles.
Tube 3 can be stored at room temperature.
Range of complex IV / cytochrome c oxidase assay kitsBiochemical assay - ab239711
Immunocapture with biochemical assay (plate-based) - ab109911 (rodent) and ab109909 (human)
Immunocapture with biochemical assay (dipstick) - ab109878 (rodent) (this kit) and ab109876 (human)
Immunocapture with biochemical assay and ELISA - ab109910 (human)
ELISA - ab179880 (human)
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アプリケーション
適用あり: Functional Studiesmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Please refer to protocols. -
内容 30 tests 96-well microplate 2 units Buffer B (10X Blocking solution) 1 x 2ml Dipsticks 1 x 30 units Extraction Buffer (ab260490) 1 x 15ml Tube 1 (DAB 100X stock) 1 x 100µl Tube 2 (reduced cytochrome c 20X stock) 1 x 500µl Tube 3 (Buffer for activity assay) 1 x 10ml Wash buffer 1 x 2ml -
研究分野
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別名
- cco
- COX
- Cytochrome c oxidase
- Cytochrome oxidase
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109878の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent dilution.
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特記事項 |
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Functional Studies
Use at an assay dependent dilution. |
画像
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Figure 2. An example using ab109878 to quantify Complex IV enzyme activity in mouse protein extracts Based on the standard curve, 50 µg of protein extract were loaded onto a dipstick for each sample. The figure shows three developed dipsticks from unknown samples (1-3). The analysis of the signal intensity and interpolation from the standard curve showed that the unknown samples have between 23-87% of normal Complex IV activity levels.
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Figure 1. An example using ab109878 to quantify Complex IV enzyme activity in mouse protein extracts Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 100 µg of protein extract.
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Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab109878 は 6 報の論文で使用されています。
- Zhang W et al. Mst1 regulates non-small cell lung cancer A549 cell apoptosis by inducing mitochondrial damage via ROCK1/F-actin pathways. Int J Oncol 53:2409-2422 (2018). PubMed: 30320378
- Kumar RP et al. Regulation of energy metabolism during early mammalian development: TEAD4 controls mitochondrial transcription. Development 145:N/A (2018). PubMed: 30201685
- Chavan H et al. Arsenite Effects on Mitochondrial Bioenergetics in Human and Mouse Primary Hepatocytes Follow a Nonlinear Dose Response. Oxid Med Cell Longev 2017:9251303 (2017). PubMed: 28163822
- Vafai SB et al. Natural Product Screening Reveals Naphthoquinone Complex I Bypass Factors. PLoS One 11:e0162686 (2016). Functional Studies . PubMed: 27622560
- Bär F et al. Mitochondrial Gene Polymorphisms That Protect Mice From Colitis. Gastroenterology N/A:N/A (2013). Cellular Activity ; Mouse . PubMed: 23872498
- Choi EM Honokiol protects osteoblastic MC3T3-E1 cells against antimycin A-induced cytotoxicity. Inflamm Res : (2011). Mouse . PubMed: 21800176