Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))
Total protein in input
5e+006 cells
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
Mouse embryonic fibroblasts (MEFs)
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce™ IP Lysis Buffer
Dilution
1/500
Additional data
Additional Notes
Lane 1: IP by control rabbit IgG (Abcam ab27472)
Lane 2: IP by collagen IV antibody (Abcam ab6586).
Band corresponding to collagen IV (220-230 kDa) can be detected in collagen IV pulldown sample but not control IgG sample.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 04 2016