Application
ELISA
Sample
Human Serum (blood)
Specification
blood
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Type
Sandwich (Capture)
Other product details
Dilution
1/10
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: mouse IgG HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
I have used this Ab in ELISA and it seemed to work in 1:200 ratio
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Yeri-Alice Rim
Verified customer
投稿 Apr 15 2013