Application
Western blot
Sample
Pig Tissue lysate - whole (skin)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount
20 µg
Specification
skin
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: donkey anti-mouse IgG-HPR
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 130 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 10 2016