Anti-Cofilin 抗体 [1A1] (ab54532)
Key features and details
- Mouse monoclonal [1A1] to Cofilin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Cofilin antibody [1A1]
Cofilin-1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1A1] to Cofilin -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Cow, Pig, Cynomolgus monkey -
免疫原
Recombinant full length protein (GST-tag) corresponding to Human Cofilin. AAH11005
Sequence:MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILE EGKEILVGDVGQTVDDPYATFAKMLPDKDCRYALYDATYETKESKKEDLV FIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEGVKDRCTLA EKLGGSAVISLEGKPL
Database link: P23528 -
ポジティブ・コントロール
- WB: HeLa, PC-12 and NIH/3T3 cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
1A1 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54532の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
組織特異性
Widely distributed in various tissues. -
配列類似性
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
翻訳後修飾
Phosphorylated on Ser-3 in resting cells. -
細胞内局在
Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. - Information by UniProt
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参照データベース
- Entrez Gene: 1072 Human
- Entrez Gene: 12631 Mouse
- Entrez Gene: 29271 Rat
- Omim: 601442 Human
- SwissProt: P23528 Human
- SwissProt: P18760 Mouse
- SwissProt: P45592 Rat
- Unigene: 170622 Human
see all -
別名
- 18 kDa phosphoprotein antibody
- CFL 1 antibody
- CFL antibody
see all
画像
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Anti-Cofilin antibody [1A1] (ab54532) at 1 µg + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 25 µg
Predicted band size: 18 kDaThis image was generated using the ascites version of the product
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Cofilin with ab54532 at 1.5 µg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab54532 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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Anti-Cofilin antibody [1A1] (ab54532) at 1 µg/ml + NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Predicted band size: 18 kDaThis image was generated using the ascites version of the product.
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Anti-Cofilin antibody [1A1] (ab54532) at 1 µg/ml + PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Predicted band size: 18 kDaThis image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (28)
ab54532 は 28 報の論文で使用されています。
- Keen AN et al. Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells. J Cell Biol 221:N/A (2022). PubMed: 35024764
- Kauer SD et al. Inositol Polyphosphate-5-Phosphatase K (Inpp5k) Enhances Sprouting of Corticospinal Tract Axons after CNS Trauma. J Neurosci 42:2190-2204 (2022). PubMed: 35135857
- Nishtala K et al. Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing. Int J Mol Sci 23:N/A (2022). PubMed: 35269714
- Feng L et al. Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway. Front Med (Lausanne) 9:861371 (2022). PubMed: 35492354
- Carrera-Cañas C et al. Plasticity of the hypocretinergic/orexinergic system after a chronic treatment with suvorexant in rats. Role of the hypocretinergic/orexinergic receptor 1 as an autoreceptor. Front Mol Neurosci 15:1013182 (2022). PubMed: 36277486