Application
Western blot
Sample
Pig Tissue lysate - whole (endometrial tissue lysate)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
endometrial tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Diluent: TBST
Concentration
1 µg/ml
Secondary antibody
Dilution
1/30000
Name
Non-Abcam antibody was used: Anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
Colorimetric
Exposure
25 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 15 kDa
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投稿 Feb 11 2016