Anti-CoREST3 / RCOR3 抗体 (ab76921)
Key features and details
- Rabbit polyclonal to CoREST3 / RCOR3
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CoREST3 / RCOR3 antibody -
製品の詳細
Rabbit polyclonal to CoREST3 / RCOR3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-P, ICCmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human CoREST3/ RCOR3. Synthetic peptide corresponding to a region between residue 445 and 495 of human CoREST3/ RCOR3 (NP_060724.1), (and between residues 503-553 NP_001129695.1.).
Database link: Q9P2K3 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76921の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200.
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ICC |
1/50 - 1/250.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200. |
ICC
1/50 - 1/250. |
ターゲット情報
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関連性
The CoREST3 gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 55758 Human
- Entrez Gene: 214742 Mouse
- SwissProt: Q9P2K3 Human
- SwissProt: Q6PGA0 Mouse
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別名
- 4921514E24Rik antibody
- C730034D20Rik antibody
- E130101E15Rik antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling CoREST3 / RCOR3 with ab76921 at 1/200 dilution.
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Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells labelling CoREST3 / RCOR3 with ab76921 at 1/80 (2.5µg/ml). A DyLight® 488-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
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All lanes : Anti-CoREST3 / RCOR3 antibody (ab76921) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 second -
Detection of CoREST3 / RCOR3 by Western Blot of Immunprecipitate.
ab76921 at 1µg/ml staining CoREST3 / RCOR3 in HeLa whole cell lysate immunoprecipitated using ab76921 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab76921 は 3 報の論文で使用されています。
- Sáez JE et al. PIAS? controls stability and facilitates SUMO-2 conjugation to CoREST family of transcriptional co-repressors. Biochem J 475:1441-1454 (2018). PubMed: 29555846
- Sáez JE et al. Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation. PLoS One 10:e0131760 (2015). PubMed: 26111147
- Barrios ÁP et al. Differential properties of transcriptional complexes formed by the CoREST family. Mol Cell Biol 34:2760-70 (2014). PubMed: 24820421