Anti-CoCoA 抗体 (ab70564)
Key features and details
- Rabbit polyclonal to CoCoA
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CoCoA antibody
CoCoA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CoCoA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human CoCoA (C terminal). Synthetic peptide corresponding to a region between residue 650 and the C terminus (residue 691) of human CoCoA (NP_065949.1)
Database link: Q9P1Z2 -
ポジティブ・コントロール
- Whole cell lysate from: HeLa, 293T, NIH3T3, Ramos and MCF7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70564 was affinity purified using an epitope specific to CoCoA immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70564の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1 mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions. -
細胞内局在
Cytoplasm. Nucleus. Note: shuttles between nucleus and cytoplasm. -
参照データベース
- Entrez Gene: 57658 Human
- Entrez Gene: 67488 Mouse
- Entrez Gene: 246047 Rat
- SwissProt: Q9P1Z2 Human
- SwissProt: Q8CGU1 Mouse
- SwissProt: Q66HR5 Rat
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別名
- Calcium binding and coiled coil domain 1 antibody
- CALCOCO1 antibody
- Calphoglin antibody
see all
画像
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All lanes : Anti-CoCoA antibody (ab70564) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Lane 4 : Ramos whole cell lysate
Lane 5 : MCF7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of human CoCoA by Immunoprecipitation in whole cell lysate from MCF7 cells (1 mg, 20% of immunoprecipitate loaded/lane), using ab70564 at 3 µg/mg of lysate (lane 2).
CoCoA was also immunoprecipitated using another CoCoA antibody (ab70563) which recognizes an upstream epitope (lane 1).
Lane 3: Control IgG immunoprecipitate.
Subsequent blotting of immunoprecipitate for the detection of CoCoA was performed using 1µg/ml of ab70564 and 50µg of lysate.
Detection was by chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab70564 は 2 報の論文で使用されています。
- Stefely JA et al. Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. Autophagy 16:2219-2237 (2020). PubMed: 31971854
- Karere GM et al. Identification of candidate genes encoding an LDL-C QTL in baboons. J Lipid Res 54:1776-85 (2013). PubMed: 23596326