Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1 M PBS with 1% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1M PBST with 10%donkeys serum
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa fluoro 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
12 M old APP transgenic mice was perfused with 4% PFA in 0.1M PBS. IFC staining performed in brain sections with rabbit polyclonal anti-ApoJ-Ab92548 with Alexa 488 and LRP2 (Ab184676)-568. ApoJ staining was visible in the chroid plexus and corpus callosum and do not co-localised with LRP2. Nucleus stained with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
投稿 Feb 29 2016