Application
Western blot
Sample
Clostridium botulinum Purified protein (cell lysate)
Loading amount
5 µg
Specification
cell lysate
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP)
Dilution
1/10000
Detection
Detection method
BCIP
Exposure
20 minute(s) and 0 second(s)
Additional data
Additional Notes
My loading sample onto 12% SDS-PAGE for WB was putified botulinum toxin type A.
Therefore, Your antibody (Ab83064) for botulinum toxin type B should not detect any band because my sample was type A.
This is the reason why there is no detected bands.
This is perfect data that I want to. Therefore, I give this Ab83064 five stars.
Thank you.
Therefore, Your antibody (Ab83064) for botulinum toxin type B should not detect any band because my sample was type A.
This is the reason why there is no detected bands.
This is perfect data that I want to. Therefore, I give this Ab83064 five stars.
Thank you.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. chung sei kim
Verified customer
投稿 Aug 01 2012