Application
Western blot
Sample
Human Cell lysate - whole cell (glioma cells)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Treatment
0.5 mM staurosporine in DMSO for 6hrs
Specification
glioma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit HRP 2nd Ab
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 90 kDa
Additional data
Additional Notes
Glioma cells were treated with apoptosis-inducing drug staurosporine to set up a positive control for future study, cleaved PARP were dramatically induced with drug treatment.
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投稿 Jun 08 2010