Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Specification
Jurkat cells
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
1 hour(s) and 30 minute(s)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
HRP
Negative control
Non-induced jurket cells (no etoposide)
Exposure
5 second(s)
Bands
Specific: 85 kDa
Positive control
Induced Jurkat cell (etoposide)
Additional data
Additional Notes
The lane marked 1 on the top is Non-induced Jurkat cells (no band visable)
The lane marked 2 on the top is Induced Jurkat cells (band visable at 85kDa)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Adam Szadkowski
Verified customer
投稿 Jan 26 2006