Anti-Cleaved PARP1 抗体 [4B5BD2] (ab110315)
Key features and details
- Mouse monoclonal [4B5BD2] to Cleaved PARP1
- Suitable for: WB, ICC/IF, In-Cell ELISA, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-Cleaved PARP1 antibody [4B5BD2]
Cleaved PARP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4B5BD2] to Cleaved PARP1 -
由来種
Mouse -
特異性
ab110315 reacts with the N-terminal end formed by the cleavage adjacent to Asp214; it thus recognizes the apoptosis-specific 89 kDa catalytic domain fragment, but it does not recognize the full-length PARP1 or the 24 kDa DNA binding domain fragment. -
アプリケーション
適用あり: WB, ICC/IF, In-Cell ELISA, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- Staurosporine-treated HeLa and HL60 cells
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特記事項
This monoclonal antibody to cleaved PARP1 has been knockout validated in Western blot. The expected band for cleaved PARP1 was observed in wild type cells and the band was not seen in knockout cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
特記事項(精製)
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by ammonium sulfate precipiation. -
ポリ/モノ
モノクローナル -
クローン名
4B5BD2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110315の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 113 kDa.
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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In-Cell ELISA |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 113 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. |
In-Cell ELISA
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. -
配列類似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻訳後修飾
Phosphorylated by PRKDC and TXK.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
細胞内局在
Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
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別名
- ADP ribosyltransferase diphtheria toxin like 1 antibody
- ADP ribosyltransferase NAD(+) antibody
- ADP-ribosyltransferase diphtheria toxin-like 1 antibody
see all
画像
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Lane 1: Wild type HAP1 (untreated) whole cell lysate (20 µg)
Lane 2: PARP1 (untreated) knockout HAP1 (untreated) whole cell lysate (20 µg)
Lane 3: HeLa (untreated) whole cell lysate (20 µg)
Lane 4: HAP1 (staurosporine treated, 1 uM, 4 hr) whole cell lysate (20 µg)
Lane 5: PARP1 (staurosporine treated, 1 uM, 4 hr) knockout HAP1 whole cell lysate (20 µg)
Lane 6: HeLa (staurosporine treated, 1 uM, 4 hr) whole cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green - ab110315 observed at 100 kDa. Red - loading control, ab181602, observed at 37 kDa
ab110315 detected the expected band for cleaved PARP1 in wild type HAP1 cells treated with staurosporine and the band was not seen in PARP1 knockout cells treated with staurosporine. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. ab110315 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry images of stained untreated (A) and 4 hours 1 µM Staurosporine-treated (B) Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with 1.0 µg/ml ab110315 for 2 hours at room temperature or over night at 4°C. 10% goat serum was used as the blocking agent for all blocking steps. The secondary antibody was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (in green) used at 2.0 µg/ml for 2 hours. DAPI was used to stain the cell nuclei (in red). Heat induced antigen retrieval (0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C) improves signal. Note that the ab110315 labels only condensed and/or fragmented nuclei of apoptotic Staurosporine-treated cells.
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Lanes 1-2 : Antibody that recognizes full-length PARP1
Lanes 3-4 : Anti-Cleaved PARP1 antibody [4B5BD2] (ab110315) at 1 µg/ml
Lanes 1 & 3 : untreated HeLa cells
Lanes 2 & 4 : HeLa cells treated with 1 µM Staurosporinefor 4 hours
Lysates/proteins at 20 µg per lane.
Predicted band size: 113 kDaWestern Blot analysis using ab110315 antibody and 20 µg of untreated (CON) or 4 hours 1 µM Staurosporine-treated (STS) HeLa cells. Blots were incubated with an antibody that recognizes both the full-length PARP1 and its 89 kDa fragment (left panel), or 1.0 µg/mL PARP1 (cleaved) antibody (ab110315) (right panel). Appropriate HRP-conjugated secondary antibodies followed by ECL detection were used. Note that the MS777 antibody recognizes the apoptosis-specific 89 kDa fragment of PARP1 but it does not recognize the full-length PARP1.
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In-Cell ELISA (ICE) using ab110315 on HeLa cells treated with Staurosporine to induce apoptosis. HeLa cells were seeded overnight (50,000 cells/well), treated for 4 hours with 1 µM Staurosporine or with the drug vehicle (DMSO), fixed for Detaching Adherent Cells and analyzed.
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Flow cytometry analysis of apoptosis using ab110315. HL-60 cells were treated with 1 µM Staurosporin for 4 hours (blue) or vehicle control (red). Control cells were also stained with an equal amount of an isotype control antibody (black).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab110315 は 10 報の論文で使用されています。
- Kashkoulinejad-Kouhi T et al. Enhancement of cisplatin sensitivity in human breast cancer MCF-7 cell line through BiP and 14-3-3? co-knockdown. Oncol Rep 45:665-679 (2021). PubMed: 33416155
- Pillai-Kastoori L et al. Antibody validation for Western blot: By the user, for the user. J Biol Chem 295:926-939 (2020). PubMed: 31819006
- Jing W et al. Artesunate promotes sensitivity to sorafenib in hepatocellular carcinoma. Biochem Biophys Res Commun 519:41-45 (2019). PubMed: 31481232
- Li W et al. Overexpression of Smac by an Armed Vesicular Stomatitis Virus Overcomes Tumor Resistance. Mol Ther Oncolytics 14:188-195 (2019). PubMed: 31312717
- Das R et al. New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules. J Cell Sci 132:N/A (2019). PubMed: 31138677
- Hou Y et al. Genetic ablation of TAZ induces HepG2 liver cancer cell apoptosis through activating the CaMKII/MIEF1 signaling pathway. Onco Targets Ther 12:1765-1779 (2019). PubMed: 30881030
- Huang D et al. Oxaliplatin and infliximab synergize to induce regression of colon cancer. Oncol Lett 15:1517-1522 (2018). PubMed: 29434844
- Mayrand D & Fradette J High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered Substitutes. Methods Mol Biol 1773:93-105 (2018). PubMed: 29687383
- Arosh JA et al. Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis. Proc Natl Acad Sci U S A 112:9716-21 (2015). IHC-Fr ; Mouse . PubMed: 26199416
- Akazawa Y et al. BH3-only protein Bim is associated with the degree of Helicobacter pylori-induced gastritis and is localized to the mitochondria of inflammatory cells in the gastric mucosa. Int J Med Microbiol 305:553-62 (2015). PubMed: 26197709