Application
Western blot
Sample
Human Cell lysate - whole cell (liver cancer)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
liver cancer
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: primary antibody dilution solution
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: HRP conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal West Dura
Exposure
40 second(s)
Bands
Specific: 25 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 09 2012