Application
Western blot
Sample
Human Cell lysate - whole cell (human urothelial cells)
Loading amount
15 µg
Specification
human urothelial cells
Treatment
5% ABS for 7 days
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Secondary antibody
Name
Non-Abcam antibody was used: molecular probes IR 800 dye
Host species: Goat
Clonality: Polyclonal
Host species: Goat
Clonality: Polyclonal
Detection
Detection method
licor odyssey
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: na kDa
Positive control
Human urothelial cells treated for 7 days with 5% ABS (+ve control)
Additional data
Additional Notes
First lane: differentiated cells (high Claudin 3)
Second lane non-differentiated cells (low Claudin 3)
Non-treated cells show reduced expression of Claudin 3 which we expect however, as there is still a band observed this could potentially be other Claudins being detected.
Second lane non-differentiated cells (low Claudin 3)
Non-treated cells show reduced expression of Claudin 3 which we expect however, as there is still a band observed this could potentially be other Claudins being detected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 16 2012