Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
No
Specification
Kidney
Blocking step
3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
3.2% PFA and 0.5% GA
Other product details
Incubation time
45 minute(s) · Temperature: 25°C · Diluent: 3% BSA and 0.5% TX100
Dilution
1/500
Secondary antibody
Concentration
2 µg/ml
Name
Non-Abcam antibody was used: Donkey anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
IF shows has high background.
Abcam response
We are sorry to hear that this antibody has not been working well in your experiment. Unfortunately, this product is not validated to work in Monkey.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Aaron Halpen
Verified customer
投稿 Sep 29 2014