This kit was purchased to see if it was compatible with mitochondria isolated from bovine PBMCs. The initial data looks good but more tests are needed to see if bovine samples can be stored at -80C for 6 months like stated in the protocol booklet. The image I attached shows the following data: A1 B1 (Blank), C1 D1 (Fresh Sample A), E1 F1 (Fresh Sample B), G1 H1 (1 month old sample C). There was some variation in the methods used to isolate the mitochondria in the fresh and 1 month old sample as we are still working to improve this part of the experiment. It is unknown if the variation in mitochondria isolation methods or the age of the samples used is responsible for the differences observed graphically.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 27 2016