Application
Western blot
Sample
Rat Cell lysate - whole cell (Colonic epithelium)
Loading amount
15 µg
Specification
Colonic epithelium
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Immunoresearch donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000
Detection
Detection method
ECL+
Exposure
2 second(s)
Bands
Specific: ~ 80-100 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Laura Wrzosek
Verified customer
投稿 Mar 31 2011