Anti-Chp1 抗体 (ab18191)
Key features and details
- Rabbit polyclonal to Chp1
- Suitable for: ChIP, WB
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
製品の概要
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製品名
Anti-Chp1 antibody -
製品の詳細
Rabbit polyclonal to Chp1 -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, WBmore details -
種交差性
交差種: Schizosaccharomyces pombe -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in S. pombe whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18191の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP | (1) |
Use 2.5µg for 107 cells.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).
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特記事項 |
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ChIP
Use 2.5µg for 107 cells. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa). |
ターゲット情報
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関連性
Chp1 a chromodomain protein associates with the S. pombe mating type locus and telomeres. Chp1 localization to heterochromatin depends on its chromodomain and the C-terminal domain of the protein. Chp1 and centromeric siRNAs are components of the RITS (RNA-induced initiation of transcriptional gene silencing) complex. The association of RITS with centromeres is linked to Dicer activity, which is required to cleave centromeric transcripts to produce short interfering RNAs that actively recruit components of heterochromatin to centromeres. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 2542239 Schizosaccharomyces pombe
- SwissProt: Q10103 Schizosaccharomyces pombe
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別名
- Chromo domain containing protein 1 antibody
- Chromodomain protein Chp1 antibody
画像
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All lanes : Anti-Chp1 antibody (ab18191) at 1 µg/ml
Lane 1 : S. pombe chp1Δ lysate
Lane 2 : S. pombe wildtype lysate
Predicted band size: 109 kDa
Observed band size: 109 kDa
Additional bands at: 102 kDa (possible degradation product)
ab18191 specifically recognises Chp1 at 109 kDa in wildtype S. pombe lysate but not in chp1Δ lysate. -
X-ChIP performed with ab18191 on Schizosaccharomyces pombe Cell lysate (whole cell). 5µl antibody were used per 500µl of chromatin/cell lysate according to standard S. pombe ChIP protocols. Protein-A Sepharose was used to recover the ChIPs. In the positive control the researcher specifically induced silencing of the ura4+ gene. For the negative control the researcher performed multiplex PCR with primers amplifying ura4+ and an un-silenced gene. In addition, act1+ was amplified in a separate PCR reaction. Chp1 only binds to ura4+ (and only if silencing is induced).
This image is courtesy of an Abreview submitted by Marc B? on 9 February 2006
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Anti-Chp1 antibody (ab18191) at 1 µg/ml + S.pombe Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 109 kDa
Observed band size: 115,75 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab18191 は 14 報の論文で使用されています。
- Gao Z et al. FK506-binding protein 5 promotes the progression of papillary thyroid carcinoma. J Int Med Res 49:3000605211008325 (2021). PubMed: 33906532
- Jih G et al. Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription. Nature 547:463-467 (2017). ChIP . PubMed: 28682306
- Zocco M et al. The Chp1 chromodomain binds the H3K9me tail and the nucleosome core to assemble heterochromatin. Cell Discov 2:16004 (2016). ChIP . PubMed: 27462451
- Creamer KM et al. The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription. Mol Cell Biol 34:2046-61 (2014). ChIP ; Schizosaccharomyces pombe . PubMed: 24662054
- Shim YS et al. Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin. EMBO J 31:4375-87 (2012). PubMed: 22990236