Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lung tumor and normal lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER Citrate Pressure Cooker
Permeabilization
No
Specification
Lung tumor and normal lung
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1M Tris/2%FBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Biotinylated Goat anti-Rabbit Vector Labs BA-1000
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin-maleimide
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin-maleimide
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sofya Osharovich
Verified customer
投稿 Mar 15 2017