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Products:Neuroscience >> Neurotransmitter >> Acetylcholine
Overview
Product name
Anti-Choline Acetyltransferase antibody
Choline Acetyltransferase 抗体 (15件) 一覧
Description
Rabbit polyclonal to Choline Acetyltransferase
Tested applications
ICC/IF, IHC-P, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide as a part of rat and mouse ChAT (choline O-acetyltransferase) conjugated to an immunogenic carrier protein.
Positive control
1 day old mouse cortical cell culture Rat spinal cord
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurotransmitter >> Acetylcholine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
ICC/IF
IHC-P
IHC-Fr
Application notes
ICC/IF: 1/2000.
IHC-P: 1/300 - 1/2000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/2000.
WB: 1/300 - 1/2000. Predicted molecular weight: 83 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Involvement in disease
Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family.
Target information above from: UniProt accessionP28329
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Choline O acetyltransferase antibody
- Choline O acetyltransferase antibody
- Acetyl CoA choline O acetyltransferase antibody
see all
Database links
- Entrez Gene: 12647 Mouse
- Entrez Gene: 290567 Rat
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 442817 Mouse
- Unigene: 45116 Rat
Anti-Choline Acetyltransferase antibody images:
Immunocytochemistry/ Immunofluorescence - Choline Acetyltransferase antibody (ab70219)
Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70219 at 1/2000 dilution. Cells are counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence - Choline Acetyltransferase antibody (ab70219)
Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70219 at 1/2000 dilution. Cells are counterstained with DAPI.
- Choline Acetyltransferase antibody (ab70219)
Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution.
- Choline Acetyltransferase antibody (ab70219)
Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution.
- Choline Acetyltransferase antibody (ab70219)
Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution.
References for Anti-Choline Acetyltransferase antibody (ab70219)
ab70219 has not yet been referenced specifically in any publications.
Publishing research using ab70219? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"