Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.25 % Triton-x ind 0.1M PB
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Biotinylated anti-rabbit (Vector labs)
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/500
Additional data
Additional Notes
Samples fixed in 4 % PFA for 4 hours. Free-floating sections cut from tissue embedded in 30 % sucrose.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 10 2013