Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 colon cancer cell line)
Loading amount
60 µg
Specification
HCT116 colon cancer cell line
Treatment
DMSO control and 10 µM etoposide for 24 hrs
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 % Milk in PBS-T buffer
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/10000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 150 kDa
Additional data
Additional Notes
Cells were treated 24 hrs with 10 µM etoposide. We recomend to use a secondary antibody from Abcam for ab85743. With another non-Abcam secondary we didn't get a good signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Christian Marx
Verified customer
投稿 Oct 26 2012