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Product Name
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Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP)
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Product type
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Secondary antibodies
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Description
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Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP)
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Immunogen
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chicken IgY whole molecule
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IHC-P, IM, WB
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Application notes
(see key)
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Recommended dilutions
suitable for immunoblotting (dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
WB: Use at an assay dependent concentration (from PubMed:16543490).
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Chicken >> IgY >> Enzyme >> HRP
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Raised in
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Goat
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Horseradish Peroxidase (HRP)
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Storage buffer
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0.01M NaPhos, 0.14M NaCl, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6877)
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This product has been used in: (two most recent references)
Wang X et al. Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol 184:143-58 (2009). WB. PubMed: 19139267
Kang Q & Chen A Curcumin suppresses expression of low-density lipoprotein (LDL) receptor, leading to the inhibition of LDL-induced activation of hepatic stellate cells. Br J Pharmacol 157:1354-67 (2009). PubMed: 19594758
See all 8 publication references for this product.
If you publish research using ab6877 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgY
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Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) - more information
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