Chicken IgY secondary antibody - H&L (ab6753)

Product Name 

Chicken IgY secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Rabbit polyclonal to Chicken IgY - H&L (HRP)

Immunogen 

chicken IgY whole molecule

Tested applications 
(see key)

Dot, ELISA, IHC-Fr, IHC-P, IM, WB

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.

suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Please refer to the relevant conjugation for dilutions specific for this product:

Research areas 

Secondary antibodies >> anti-Chicken >> IgY >> Enzyme >> HRP

Relevance 

Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Conjugation notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

Storage buffer 

Preservative: 0.01% Gentamicin Sulfate (Do not add Sodium Azide!)
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。

Purification notes 

Horseradish Peroxidase (HRP).

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

1.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Chicken IgY secondary antibody - H&L (ab6753)

  This product has been used in: (two most recent references)

Vargas NB et al. Protein kinase C activation stabilizes LDL receptor mRNA via the JNK pathway in HepG2 cells. J Lipid Res 50:386-97 (2009). PubMed: 18936517

Papalouka V et al. Muscle LIM protein interacts with cofilin 2 and regulates F-actin dynamics in cardiac and skeletal muscle. Mol Cell Biol 29:6046-58 (2009). WB. PubMed: 19752190

See all 10 publication references for this product.


If you publish research using ab6753 please let us know so that we can cite the reference on this datasheet.

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