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Product Name
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Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (TR)
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (TR)
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Immunogen
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chicken IgY whole molecule
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IM
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution (PMID 18482827).
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Chicken >> IgY >> Fluorophore >> Texas Red
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。
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Purification notes
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Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons)
Absorption Wavelength: 596 nm
Emission Wavelength: 620 nm
Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
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Form
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Liquid
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Conjugation
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Texas Red ®
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Concentration
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1.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (TR) について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (TR) (ab6751)
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This product has been used in: (two most recent references)
Myhnen TT et al. Localization of prolyl oligopeptidase in the thalamic and cortical projection neurons: a retrograde neurotracing study in the rat brain. Neurosci Lett 450:201-5 (2009). IHC-FrFl. PubMed: 19041368
Crump D et al. Detection of PBDE effects on mRNA expression in chicken (Gallus domesticus) neuronal cells using real-time RT-PCR and a new differential display method. Toxicol In Vitro 22:1337-43 (2008). ICC/IF. PubMed: 18482827
If you publish research using ab6751 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgY
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