Chicken IgY secondary antibody - H&L (ab6749)

Product Name 

Chicken IgY secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Rabbit polyclonal to Chicken IgY - H&L (FITC)

Immunogen 

chicken IgY whole molecule

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IM

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution (PMID 18983843).
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Please refer to the relevant conjugation for dilutions specific for this product:

Research areas 

Secondary antibodies >> anti-Chicken >> IgY >> Fluorophore >> FITC

Relevance 

Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons)
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

1.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Chicken IgY secondary antibody - H&L (ab6749)

  This product has been used in: (two most recent references)

Kuo YC et al. Capillary electrophoresis of bone marrow stromal cells with uptake of heparin-functionalized poly(lactide-co-glycolide) nanoparticles during differentiation towards neurons. Electrophoresis 31:315-23 (2010). ICC/IF. PubMed: 20084630

Tomasi ML et al. S-adenosylmethionine regulates apurinic/apyrimidinic endonuclease 1 stability: implication in hepatocarcinogenesis. Gastroenterology 136:1025-36 (2009). ICC/IF. PubMed: 18983843

See all 3 publication references for this product.


If you publish research using ab6749 please let us know so that we can cite the reference on this datasheet.

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