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Products:Secondary antibodies >> anti-Chicken >> IgY >> Biotin
Overview
Product name
Goat anti-Chicken IgY H&L (Biotin) secondary antibody
IgY 抗体 (89件) 一覧
Description
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin)
Target species
Chicken
Tested applications
ICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
Immunogen
chicken IgY whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgY fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Chicken >> IgY >> Biotin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/500 - 1/3000.ICC/IF: 1/500 - 1/3000.
Dot: Use at an assay dependent concentration.
ELISA
ELISA: 1/300000ELISA: 1/300000
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
WB
WB: 1/3000 - 1/30000.WB: 1/3000 - 1/30000.
References for Goat anti-Chicken IgY H&L (Biotin) secondary antibody (ab6876)
This product has been referenced in:
- Yang Zet al. {beta}-Agonist-associated Reduction in RGS5 Expression Promotes Airway Smooth Muscle Hyper-responsiveness. J Biol Chem 286:11444-55 (2011). IHC-Fr; Human.Read more (PubMed: 21278382) »
See 1 publication for this product
Publishing research using ab6876? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"