Anti-CNTLN 抗体 (ab122356)
Key features and details
- Rabbit polyclonal to CNTLN
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CNTLN antibody -
製品の詳細
Rabbit polyclonal to CNTLN -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human CNTLN aa 289-379.
Sequence:LIEARKEVEVSQSKYNALSLQLSNKQTELIQKDMDITLVRKELQELQNLY KQNSTHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESIS
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ポジティブ・コントロール
- Human liver tissue; Human liver lysate; Human cell line U-251 MG
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122356の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
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特記事項 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
ターゲット情報
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細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Colocalizes with gamma-tubulin during interphase and mitosis. Appears to associated with the mother centriole during G1 phase and with daughter centrioles towards G1/S phase. - Information by UniProt
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参照データベース
- Entrez Gene: 54875 Human
- Omim: 611870 Human
- SwissProt: Q9NXG0 Human
- Unigene: 435381 Human
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別名
- bA340N12.1 antibody
- C9orf101 antibody
- C9orf39 antibody
see all
画像
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ab122356, at 1/60 dilution, staining CNTLN in paraffin-embedded Human liver tissue by Immunohistochemistry.
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All lanes : Anti-CNTLN antibody (ab122356) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate -
ab122356, at 4 µg/ml, staining CNTLN in Human cell line U-251 MG by Immunofluorescence. Antibody staining is shown in green.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab122356 は論文での使用が確認できていません。