Anti-Cellular Apoptosis Susceptibility/CSE1L 抗体 (ab70547)
Key features and details
- Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cellular Apoptosis Susceptibility/CSE1L antibody
Cellular Apoptosis Susceptibility/CSE1L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to Human Cellular Apoptosis Susceptibility/CSE1L (N terminal).
Database link: NP_001307.2 -
ポジティブ・コントロール
- WB: HeLa and Ramos whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Human testicular seminoma; mouse plasmacytoma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70547の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/10000 - 1/25000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/25000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
組織特異性
Highly expressed in proliferating cells. -
配列類似性
Belongs to the XPO2/CSE1 family.
Contains 1 importin N-terminal domain. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 419212 Chicken
- Entrez Gene: 518622 Cow
- Entrez Gene: 1434 Human
- Entrez Gene: 110750 Mouse
- Entrez Gene: 362273 Rat
- Omim: 601342 Human
- SwissProt: P55060 Human
- SwissProt: Q9ERK4 Mouse
see all -
別名
- CAS antibody
- Cellular apoptosis susceptibility protein antibody
- Chromosome segregation 1 (yeast homolog) like antibody
see all
画像
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All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab70547) at 1/1000 dilution
Lane 1 : HeLa cell lysate at 20 µg
Lane 2 : HeLa Pulldown with ab70547 cell lysate at 10 µl
Lane 3 : HeLa Pulldown with ab172730 cell lysate at 10 µl
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Cellular Apoptosis Susceptibility/CSE1L antibody staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab70547 was shown to bind specifically to Cellular Apoptosis Susceptibility/CSE1L. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 30 seconds exposure time. Secondary antibodies used were VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution.
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All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab70547) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from Ramos cells at 50 µg
Developed using the ECL technique.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes -
Paraffin embedded mouse plasmacytoma tissue stained for CSE1L using ab70547 at 0.2 µg/ml in immunohistochemical analysis.
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Paraffin embedded human testicular seminoma tissue stained for CSE1L using ab70547 at 0.2 µg/ml in immunohistochemical analysis.
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Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.
Affinity purified rabbit anti-CSE1 antibody used for IP at 6 µg per reaction. CSE1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-CSE1 antibody BL1977.
For blotting immunoprecipitated CSE1, ab70547 was used at 1 µg/ml. Chemiluminescence had an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab70547 は論文での使用が確認できていません。