Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Loading amount
35 µg
Specification
HeLa Cells
Gel Running Conditions
Reduced Denaturing (8 % SDS gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 0.05% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody (Cell Signali
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/8000
Detection
Detection method
Novex ECL Chemiluminescent Substrate
Exposure
10 second(s)
Bands
Specific: 45 kDa
Additional data
Additional Notes
The background below the bands appears because the PVDF membrane was cut too close to the band on interest.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 04 2013