Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))
Loading amount
0.5 µg
Specification
Xenopus laevis gastrula embryo extract (Stage12)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Other product details
Dilution
1/500
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG Alexa Fluor 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
LI-COR ODYSSEY imaging system
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 50, 20, etc kDa
Additional data
Additional Notes
A band around the expected size (43kDa) appeared, but several additional bands are also detected.
Abcam response
Thanks for your valuable feedback. ab6544 doesn't seem to recognise the Xenopus homologue of Cdk9 under these experimental conditions. This might be due to limited homology among the human and xenopus proteins in the region where the immunogen was designed. We hope this information is useful to other users.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kei Miyamoto
Verified customer
投稿 Oct 10 2012