Anti-Cdk9 抗体 [EP3118] (ab75848)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3118] to Cdk9
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Cdk9 antibody [EP3118]
Cdk9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP3118] to Cdk9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- HT-29, HeLa and 293T cell lysates. Human breast carcinoma tissue.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP3118 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75848の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | ||
IHC-P |
特記事項 |
---|
WB: 1/2000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Is unsuitable for Flow Cyt, ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
-
機能
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 1025 Human
- Omim: 603251 Human
- SwissProt: P50750 Human
- Unigene: 150423 Human
- Unigene: 706809 Human
-
別名
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
see all
画像
-
ab75848 at 1/100 dilution staining Cdk9 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-Cdk9 antibody [EP3118] (ab75848) at 1/2000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab75848 は 2 報の論文で使用されています。
- Izumi K et al. Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin. Nat Genet 47:338-44 (2015). PubMed: 25730767
- Chen XX et al. Cyclin-dependent kinase inhibitor dinaciclib potently synergizes with cisplatin in preclinical models of ovarian cancer. Oncotarget 6:14926-39 (2015). PubMed: 25962959