Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa cell line)
Specification
HeLa cell line
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS+5%BSA
Dilution
1/1000
Detection
Detection method
ECL+
Bands
Specific: 35 kDa Non-specific: 11 kDa
Exposure
40 minute(s) and 0 second(s)
Positive control
HeLa Cells
Additional data
Additional Notes
Lanes 1 and 2: HeLa cells transfected with siRNA against Luciferase
Lanes 3 and 4: HeLa cells transfected with siRNA against human CDK5
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 12 2013