Anti-Cdk4 抗体 (ab137675)
Key features and details
- Rabbit polyclonal to Cdk4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cdk4 antibody
Cdk4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdk4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Pig -
免疫原
Recombinant fragment corresponding to Human Cdk4 aa 148-303.
Database link: P11802 -
ポジティブ・コントロール
- WB: NIH/3T3, HEK-293T, HeLa, PC-12 whole cell lysates. Epithelial OVCAR whole cell lysate. ICC/IF: HeLa and MCF7 cells. IHC-P: Human gastric carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137675の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
関連疾患
Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B. -
細胞内局在
Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1019 Human
- Entrez Gene: 12567 Mouse
- Entrez Gene: 100144492 Pig
- Entrez Gene: 94201 Rat
- Omim: 123829 Human
- SwissProt: P11802 Human
- SwissProt: P30285 Mouse
- SwissProt: P79432 Pig
see all -
別名
- Cdk 4 antibody
- cdk4 antibody
- CDK4 protein antibody
see all
画像
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HeLa cells stained for CDK4 (green) using ab137675 at 1/500 dilution in ICC/IF.
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Anti-Cdk4 antibody (ab137675) at 1/1000 dilution + HeLa whole cell extract at 30 µg
Predicted band size: 34 kDa12% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling Cdk4 using ab137675 at 1/500 dilution.
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Anti-Cdk4 antibody (ab137675) at 1/500 dilution + PC-12 whole cell extract at 30 µg
Predicted band size: 34 kDa12% SDS-PAGE
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MCF7 cells stained for CDK4 (green) using ab137675 at 1/1000 dilution in ICC/IF. Red staining of p21 Cip1 (a nucleus marker) at 1/500 dilution and Hoechst 33342 (blue) staining.
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Anti-Cdk4 antibody (ab137675) at 1/1000 dilution + HEK-293T whole cell lysate at 30 µg
Predicted band size: 34 kDa
12% SDS PAGE -
Anti-Cdk4 antibody (ab137675) at 1/1000 dilution + NIH/3T3 whole cell lysate at 30 µg
Predicted band size: 34 kDa
12% SDS PAGE -
All lanes : Anti-Cdk4 antibody (ab137675) at 1/1000 dilution
Lane 1 : Epithelial OVCAR whole cell lysate - untreated
Lane 2 : Epithelial OVCAR whole cell lysate treated with 100µM Perifosine for 24 hours
Lane 3 : Epithelial OVCAR whole cell lysate treated with 200µM Perifosine for 24 hours
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes10% SDS-PAGE.
Blocked with 5% milk for 12 hours at 4°C.
Incubated with the primary antibody in 2% milk for 12 hours at 4°C.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (70)
ab137675 は 70 報の論文で使用されています。
- Xu X et al. Proteomics analysis reveals the correlation of programmed ROS-autophagy loop and dysregulated G1/S checkpoint with imatinib resistance in chronic myeloid leukemia cells. Proteomics 22:e2100094 (2022). PubMed: 34564948
- Chen C et al. ASF1b is a novel prognostic predictor associated with cell cycle signaling pathway in gastric cancer. J Cancer 13:1985-2000 (2022). PubMed: 35399734
- Liu M et al. USP18 contributes to the proliferation and migration of ovarian cancer cells by regulating the AKT/mTOR signaling pathway. Acta Biochim Pol 69:417-422 (2022). PubMed: 35696596
- Chen J et al. S-3'-hydroxy-7', 2', 4'-trimethoxyisoxane, a novel ferroptosis inducer, promotes NSCLC cell death through inhibiting Nrf2/HO-1 signaling pathway. Front Pharmacol 13:973611 (2022). PubMed: 36105203
- Lee YP et al. Cordycepin enhances radiosensitivity to induce apoptosis through cell cycle arrest, caspase pathway and ER stress in MA-10 mouse Leydig tumor cells. Am J Cancer Res 12:3601-3624 (2022). PubMed: 36119830