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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-Cdc7 Kinase antibody
Cdc7 Kinase 抗体 (10件) 一覧
Description
Rabbit polyclonal to Cdc7 Kinase
Specificity
ab69950 detects endogenous levels of total Cdc7 Kinase protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from an internal sequence of human Cdc7 Kinase
Positive control
Jurkat cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab69950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Western blot - Cdc7 Kinase antibody (ab69950)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
Contains 1 protein kinase domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00311
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc 7 antibody
- Cdc7 antibody
- CDC7 cell division cycle 7 antibody
see all
Database links
- Entrez Gene: 8317 Human
- Omim: 603311 Human
- SwissProt: O00311 Human
- Unigene: 28853 Human
- Unigene: 533573 Human
Anti-Cdc7 Kinase antibody images:
Western blot - Cdc7 Kinase antibody (ab69950)
All lanes : Anti-Cdc7 Kinase antibody (ab69950) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 64 kDa
Observed band size : 64 kDa
References for Anti-Cdc7 Kinase antibody (ab69950)
ab69950 has not yet been referenced specifically in any publications.
Publishing research using ab69950? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"