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Western blot - Cdc7 Kinase antibody (ab69950)

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Product Name
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Cdc7 Kinase antibody
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Cdc7 Kinase 抗体 (8件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdc7 Kinase
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Immunogen
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Synthetic peptide derived from an internal sequence of human Cdc7 Kinase
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Reacts with
(species key)
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Hu
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Specificity
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ab69950 detects endogenous levels of total Cdc7 Kinase protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/5000. WB: 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell extracts.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Relevance
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DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.
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Cdc7 Kinase antibody (ab69950)の別名
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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ab69950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Abcam では全ての製品情報を一元管理しており、 Cdc7 Kinase antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for Cdc7 Kinase antibody images, references, products related to ab69950 and other tools.
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Cdc7 Kinase antibody images:
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Western blot - Cdc7 Kinase antibody (ab69950)
All lanes : Cdc7 Kinase antibody (ab69950) at 1/500 dilution
Lane 1 : Jurkat cell lysate Lane 2 : Jurkat cell lysate with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 64 kDa Observed band size : 64 kDa
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Search PubMed (MEDLINE) for references to Cdc7 Kinase
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Cdc7 Kinase antibody - more information
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Customer reviews (feedback) regarding Cdc7 Kinase antibody |
Customer FAQs regarding Cdc7 Kinase antibody |
Protocols for Cdc7 Kinase antibody |
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Cdc7 Kinase antibody - related products:
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Cdc7 Kinase antibody - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"