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Read our guarantee »Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Anti-Cdc7 Kinase antibody
Cdc7 Kinase 抗体 (10件) 一覧
Rabbit polyclonal to Cdc7 Kinase
ab69950 detects endogenous levels of total Cdc7 Kinase protein.
WB, ELISAmore details
Reacts with
Human
Synthetic peptide derived from an internal sequence of human Cdc7 Kinase
Jurkat cell extracts.
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
ab69950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Western blot - Cdc7 Kinase antibody (ab69950)
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Our Abpromise guarantee covers the use of ab69950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ELISA: 1/5000
Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
Contains 1 protein kinase domain.
Nucleus.
Target information above from: UniProt accessionO00311
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Cdc7 Kinase antibody (ab69950)

All lanes : Anti-Cdc7 Kinase antibody (ab69950) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 64 kDa
Observed band size : 64 kDa
ab69950 has not yet been referenced specifically in any publications.
Publishing research using ab69950? Please let us know so that we can cite the reference in this datasheet
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