Cdc7 Kinase antibody [DCS-341] (ab10535)

Product Name 

Cdc7 Kinase antibody [DCS-341]

Product type 

Primary antibodies

Description 

Mouse monoclonal [DCS-341] to Cdc7 Kinase

Immunogen 

Full length protein (Human).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, ICC, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 64 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A431 whole cell lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other

Relevance 

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DCS-341

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.900 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Cdc7 Kinase antibody [DCS-341] (ab10535)

  This product has been used in: (two most recent references)

Heffernan TP et al. Cdc7-Dbf4 and the human S checkpoint response to UVC. J Biol Chem 282:9458-68 (2007). PubMed: 17276990

Nambiar S et al. Identification and functional characterization of ASK/Dbf4, a novel cell survival gene in cutaneous melanoma with prognostic relevance. Carcinogenesis 28:2501-10 (2007). PubMed: 17768177
If you publish research using ab10535 please let us know so that we can cite the reference on this datasheet.

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