![Western blot - Anti-Cdc7 Kinase antibody [DCS-341] (ab10535) image](#Cdc7-Kinase-Primary-antibodies-ab10535-2.jpg)
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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-Cdc7 Kinase antibody [DCS-341]
Cdc7 Kinase 抗体 (10件) 一覧
Description
Mouse monoclonal [DCS-341] to Cdc7 Kinase
Tested applications
ELISA, WB, IP, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human).
Positive control
A431 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
DCS-341
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Cdks
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Western blot - Anti-Cdc7 Kinase antibody [DCS-341] (ab10535)
![Western blot - Anti-Cdc7 Kinase antibody [DCS-341] (ab10535) image](/ps/datasheet/images/10/ab10535/Cdc7-Kinase-Primary-antibodies-ab10535-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab10535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 0.4 µg/mlDetects a band of approximately 55 kDa (predicted molecular weight: 64 kDa).
IP: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Target
Function
Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
Contains 1 protein kinase domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00311
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc 7 antibody
- CDC7 antibody
- CDC7 cell division cycle 7 antibody
see all
Database links
- Entrez Gene: 8317 Human
- Omim: 603311 Human
- SwissProt: O00311 Human
- Unigene: 28853 Human
- Unigene: 533573 Human
Anti-Cdc7 Kinase antibody [DCS-341] images:
Western blot - Anti-Cdc7 Kinase antibody [DCS-341] (ab10535)
Predicted band size : 64 kDa
FLAG-Dbf4 forms a complex with endogenous Cdc7 following DNA damage. HeLa cells were transiently transfected with a FLAG-Dbf4 expression vector. 48 hours later cells were treated with 8 J/m2 UVC, 10 μm etoposide, or 2 mm HU. Cells were harvested 1 hour later and FLAG-Dbf4 immunoprecipitated with anti-FLAG-agarose (NS corresponds to immunoprecipitation with normal mouse IgG). The effect of DNA damage on Cdc7-Dbf4 complex formation was measured by Western immunoblot analysis using ab10535.
Image from Heffernan TP et al, J Biol Chem. 2007 Mar 30;282(13):9458-68. Epub 2007 Feb 2, Fig 7, DOI 10.1074/jbc.M611292200
References for Anti-Cdc7 Kinase antibody [DCS-341] (ab10535)
This product has been referenced in:
- Petermann Eet al. Chk1 promotes replication fork progression by controlling replication initiation. Proc Natl Acad Sci U S A 107:16090-5 (2010). WB; Human.Read more (PubMed: 20805465) »
- Heffernan TPet al. Cdc7-Dbf4 and the human S checkpoint response to UVC. J Biol Chem 282:9458-68 (2007).Read more (PubMed: 17276990) »
See all 3 publications for this product
Publishing research using ab10535? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"