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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-Cdc6 (phospho S54) antibody
Cdc6 抗体 (11件) 一覧
Description
Rabbit polyclonal to Cdc6 (phospho S54)
Specificity
ab53689 detects endogenous levels of Cdc6 only when phosphorylated at serine 54.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic phosphopeptide derived from human Cdc6 around the phosphorylation site of serine 54 (P-L-SP-P-R).
Positive control
COS7 cell extracts treated with EGF (200ng/ml, 30 mins).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Western blot - Cdc6 (phospho S54) antibody (ab53689)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Sequence similarities
Belongs to the CDC6/cdc18 family.
Cellular localization
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
Target information above from: UniProt accessionQ99741
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
see all
Database links
- Entrez Gene: 990 Human
- Entrez Gene: 23834 Mouse
- Omim: 602627 Human
- SwissProt: Q99741 Human
- SwissProt: O89033 Mouse
- Unigene: 405958 Human
- Unigene: 20912 Mouse
Anti-Cdc6 (phospho S54) antibody images:
Western blot - Cdc6 (phospho S54) antibody (ab53689)
All lanes : Anti-Cdc6 (phospho S54) antibody (ab53689) at 1/500 dilution
Lane 1 : COS7 cell extract treated
with EGF (200ng/ml, 30 mins)
Lane 2 : COS7 cell extract treated
with EGF (200ng/ml, 30 mins) with immunising peptide
Predicted band size : 63 kDa
Observed band size : 63 kDa
References for Anti-Cdc6 (phospho S54) antibody (ab53689)
ab53689 has not yet been referenced specifically in any publications.
Publishing research using ab53689? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"