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Western blot - Cdc6 (phospho S54) antibody (ab53689)
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Product Name
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Cdc6 (phospho S54) antibody
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Cdc6 抗体 (9件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cdc6 (phospho S54)
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Immunogen
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Synthetic phosphopeptide derived from human Cdc6 around the phosphorylation site of serine 54 (P-L-SP-P-R).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab53689 detects endogenous levels of Cdc6 only when phosphorylated at serine 54.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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COS7 cell extracts treated with EGF (200ng/ml, 30 mins).
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Cellular localization
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The protein is nuclear in G1 and cytoplasmic in S-phase cells.
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Research areas
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Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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Cell cycle events are regulated by the sequential activation and deactivation of cyclin depended kinase (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc6 is essential for assembling the pre-replicative complex that forms at origins of DNA replication in the G1 phase of the cell cycle in budding yeast (S. cerevisiae). Cdc6 is also involved in checkpoint controls that ensure that DNA replication is completed before initiation of mitosis.
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Cdc6 (phospho S54) antibody (ab53689)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab53689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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See below for Cdc6 (phospho S54) antibody images, references, products related to ab53689 and other tools.
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Cdc6 (phospho S54) antibody images:
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 Western blot - Cdc6 (phospho S54) antibody (ab53689)
All lanes : Cdc6 (phospho S54) antibody (ab53689) at 1/500 dilution
Lane 1 : COS7 cell extract treated
with EGF (200ng/ml, 30 mins)
Lane 2 : COS7 cell extract treated
with EGF (200ng/ml, 30 mins) with immunising peptide
Predicted band size : 63 kDa
Observed band size : 63 kDa
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Search PubMed (MEDLINE) for references to Cdc6
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Cdc6 (phospho S54) antibody - more information
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