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All lanes : Anti-Cdc6 (phospho S54) antibody (ab53689) at 1/500 dilution
Lane 1 : COS7 cell extract treated
with EGF (200ng/ml, 30 mins)
Lane 2 : COS7 cell extract treated
with EGF (200ng/ml, 30 mins) with immunising peptide
Predicted band size : 63 kDa
Observed band size : 63 kDa
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