|
|
|
|
Western blot - Cdc6 antibody [SPM380] (ab64074)
![Western blot - Cdc6 antibody [SPM380] (ab64074)](/ps/datasheet/images/ab64074-1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
Cdc6 antibody [SPM380]
|
| |
Cdc6 抗体 (9件) 一覧
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [SPM380] to Cdc6
|
|
Immunogen
|
Recombinant human Cdc6 protein.
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IP: Use at 2µg/mg of lysate.
WB: Use at a concentration of 2.5 - 5.0 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Raji cells and lysate and MDA231 cells.
|
|
Cellular localization
|
The protein is nuclear in G1 and cytoplasmic in S-phase cells.
|
|
Research areas
|
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
|
|
Relevance
|
Cell cycle events are regulated by the sequential activation and deactivation of cyclin depended kinase (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc6 is essential for assembling the pre-replicative complex that forms at origins of DNA replication in the G1 phase of the cell cycle in budding yeast (S. cerevisiae). Cdc6 is also involved in checkpoint controls that ensure that DNA replication is completed before initiation of mitosis.
|
|
|
Cdc6 antibody [SPM380] (ab64074)の別名 |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
SPM380
|
|
Isotype
|
IgG1
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
ab64074 was purified from ascites fluid by protein G.
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
Abcam では全ての製品情報を一元管理しており、 Cdc6 antibody [SPM380] について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
 |
|
|
See below for Cdc6 antibody [SPM380] images, references, products related to ab64074 and other tools.
|
| |
Cdc6 antibody [SPM380] images:
|
 Western blot - Cdc6 antibody [SPM380] (ab64074)
Cdc6 antibody [SPM380] (ab64074) at 2.5 µg/ml + Raji cell lysate at 15 µg
Predicted band size : 63 kDa
Observed band size : 63 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to Cdc6
|
| |
Cdc6 antibody [SPM380] - more information
|
Customer reviews (feedback) regarding Cdc6 antibody [SPM380] |
Customer FAQs regarding Cdc6 antibody [SPM380] |
Protocols for Cdc6 antibody [SPM380] |
Price and availability of products related to Cdc6 antibody [SPM380] |
| |
Cdc6 antibody [SPM380] - related products:
|
|
|
| |
Cdc6 antibody [SPM380] - other tools:
|
Contact Abcam with a Technical Enquiry about Cdc6 antibody [SPM380] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - Cdc6 antibody [SPM380] (ab64074)](/index.html?datasheet=64074#ab64074-1.jpg)
