![Western blot - Cdc6 antibody [SPM380] (ab64074) image](#ab64074-1.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-Cdc6 antibody [SPM380]
Cdc6 抗体 (11件) 一覧
Description
Mouse monoclonal [SPM380] to Cdc6
Tested applications
WB, IP, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human Cdc6 protein.
Positive control
Raji cells and lysate and MDA231 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab64074 was purified from ascites fluid by protein G.
Clonality
Monoclonal
Clone number
SPM380
Isotype
IgG1
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Western blot - Cdc6 antibody [SPM380] (ab64074)
![Western blot - Cdc6 antibody [SPM380] (ab64074) image](/ps/datasheet/Images/64/ab64074/ab64074-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2.5 - 5.0 µg/ml.Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP: Use at 2 µg/mg of lysate.
ELISA: Use a concentration of 0.1 - 1.0 µg/ml.
Target
Function
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Sequence similarities
Belongs to the CDC6/cdc18 family.
Cellular localization
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
Target information above from: UniProt accessionQ99741
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
see all
Database links
- Entrez Gene: 990 Human
- Omim: 602627 Human
- SwissProt: Q99741 Human
- Unigene: 405958 Human
Anti-Cdc6 antibody [SPM380] images:
Western blot - Cdc6 antibody [SPM380] (ab64074)
Anti-Cdc6 antibody [SPM380] (ab64074) at 2.5 µg/ml + Raji cell lysate at 15 µg
Predicted band size : 63 kDa
Observed band size : 63 kDa
References for Anti-Cdc6 antibody [SPM380] (ab64074)
ab64074 has not yet been referenced specifically in any publications.
Publishing research using ab64074? Please let us know so that we can cite the reference in this datasheet
![Cdc6 antibody [SPM380] for ICC/IF in Human (64074)](/ps/datasheet/images/64/ab64074/Cdc6-Primary-antibodies-ab64074-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"