Cdc6 antibody [DCS-180] (ab3220)

Product Name 

Cdc6 antibody [DCS-180]

Product type 

Primary antibodies

Description 

Mouse monoclonal [DCS-180] to Cdc6

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Immunoprecipitation (native and denatured): use at a concentration of 2µg / mg.
Western Blotting: use at a concentration of 1 - 2µg / ml for 2hrs at RT.
Detects a band of approximately 62 kDa (predicted molecular weight: 63 kDa).

This antibody does not seem suitable for ICC/IF. Please refer to abreview for further details.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Raji or MDA231 cells.

Cellular localization 

The protein is nuclear in G1 and cytoplasmic in S-phase cells.

Research areas 

Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Relevance 

Cell cycle events are regulated by the sequential activation and deactivation of cyclin depended kinase (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc6 is essential for assembling the pre-replicative complex that forms at origins of DNA replication in the G1 phase of the cell cycle in budding yeast (S. cerevisiae). Cdc6 is also involved in checkpoint controls that ensure that DNA replication is completed before initiation of mitosis.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DCS-180

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

References for Cdc6 antibody [DCS-180] (ab3220)

  This product has been used in:

Crevel G et al. The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells. PLoS ONE 3:e0001737 (2008). Human. PubMed: 18320024

If you publish research using ab3220 please let us know so that we can cite the reference on this datasheet.

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