HRP Anti-Cdc37 (phospho S13) 抗体 [EPR4879] (ab202364)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-Cdc37 (phospho S13) antibody [EPR4879]
Cdc37 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13) -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa whole cell lysate and HeLa whole cell lysate serum starved and treated with Calyculin A.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4879 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab202364の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
ターゲット情報
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機能
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. -
配列類似性
Belongs to the CDC37 family. -
翻訳後修飾
Constitutively sumoylated by UBE2I. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 11140 Human
- Entrez Gene: 12539 Mouse
- Entrez Gene: 114562 Rat
- Omim: 605065 Human
- SwissProt: Q16543 Human
- SwissProt: Q61081 Mouse
- SwissProt: Q63692 Rat
- Unigene: 160958 Human
see all -
別名
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
see all
画像
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All lanes : HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364) at 1/5000 dilution
Lane 1 : Untreated HeLa (Human epithelial carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) whole cell lysate serum starved and Calyculin A treated
Lane 3 : HeLa (Human epithelial carcinoma cell line) whole cell lysate, serum starved and Calyculin A treated. The membrane was then incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202864 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab202364 は論文での使用が確認できていません。