Anti-Cdc37 抗体 [EPR4935] (ab108305)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4935] to Cdc37
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cdc37 antibody [EPR4935]
Cdc37 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4935] to Cdc37 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- K562 cell lysate, Jurkat cell lysate, LnCaP cell lysate, SW480 cell lysate, human brain tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4935 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108305の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50000 - 1/200000. Predicted molecular weight: 44 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
perform antigen retrieval |
特記事項 |
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WB
1/50000 - 1/200000. Predicted molecular weight: 44 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. perform antigen retrieval |
ターゲット情報
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機能
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. -
配列類似性
Belongs to the CDC37 family. -
翻訳後修飾
Constitutively sumoylated by UBE2I. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 11140 Human
- Omim: 605065 Human
- SwissProt: Q16543 Human
- Unigene: 160958 Human
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別名
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
see all
画像
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All lanes : Anti-Cdc37 antibody [EPR4935] (ab108305) at 1/50000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDa -
ab108305, at 1/250, staining Cdc37 in human brain tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108305 は 1 報の論文で使用されています。
- Tirosh O et al. The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions. PLoS Pathog 11:e1005288 (2015). WB . PubMed: 26599541