Anti-Cdc27 抗体 (ab72214)
Key features and details
- Rabbit polyclonal to Cdc27
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cdc27 antibody
Cdc27 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdc27 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 225 and 275 of human Cdc27 (NP_001247.2).
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ポジティブ・コントロール
- Whole cell lysate from HeLa and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72214の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 92 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats. -
翻訳後修飾
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 540660 Cow
- Entrez Gene: 996 Human
- Entrez Gene: 217232 Mouse
- Entrez Gene: 360643 Rat
- Omim: 116946 Human
- SwissProt: A7Z061 Cow
- SwissProt: P30260 Human
- SwissProt: A2A6Q5 Mouse
see all -
別名
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
see all
画像
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All lanes : Anti-Cdc27 antibody (ab72214) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands. -
Detection of Human Cdc27 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72214 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab72214 は 1 報の論文で使用されています。
- Ren YQ et al. MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27. Med Sci Monit 21:1297-303 (2015). WB ; Human . PubMed: 25943633